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| カタログ番号 | HY280010 |
|---|---|
| 説明 |
InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077) [Iv0077] (HY280010) is a human monoclonal antibody detecting HC11 in ELISA, FCM, Neutralization. Suitable for Human.
Highlights
|
| 種反応性 | Human |
| アプリケーション | ELISA, WB, FCM, Neutralization |
| 宿主種 | Human |
| クローン性 | Monoclonal |
| アイソタイプ | IgG1, kappa |
| クローンID | Iv0077 |
| ターゲット | HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2 |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 関連製品 | pH 7.4 Dilution Buffer |
| 精製 | Protein A/G purified from cell culture supernatant. |
| アクセッション番号 | P13500 |
| 形態 | Liquid |
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 注意事項 | For research use only. Not suitable for clinical or therapeutic use. |
| Isotype Control | Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507) |
SEC-HPLC detection for InVivoMAb Anti-Human CCL2/MCP-1 (ABV0269).
Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CCL2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077).
Western blot analysis was performed using anti-CCL2/MCP-1 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 50ng
Lane 2: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 30ng
Lane 3: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 20ng
Lane 4: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 10ng
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