InVivoMAb Anti-Human CCL2/MCP-1 Antibody (Iv0077) (HY280010)

Name: InVivoMAb Anti-Human CCL2/MCP-1 Antibody (Iv0077)
Brand: abinScience
SKU: HY280010
Price: 370 USD
Availability: InStock

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Спецификация:

100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HY280010
Описание	InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077) [Iv0077] (HY280010) is a human monoclonal antibody detecting HC11 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Реактивность видов	Human
Применения	ELISA, WB, FCM, Neutralization
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1, kappa
ID клона	Iv0077
Цель	HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Связанные продукты	pH 7.4 Dilution Buffer
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P13500
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Примечание	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Изображения

Bioactivity

SEC-HPLC detection for InVivoMAb Anti-Human CCL2/MCP-1 (ABV0269).
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077).
Western blot

Western blot analysis was performed using anti-CCL2/MCP-1 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 50ng
Lane 2: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 30ng
Lane 3: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 20ng
Lane 4: recombinant human CCL2/MCP-1 (Catalog No: HY280012), 10ng