InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181) (HV514010)

Name: InVivoMAb Anti-Human R-Spondin 3 Antibody (Iv0181)
Brand: abinScience
SKU: HV514010
Price: 370 USD
Availability: InStock

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100ug
1mg

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Numéro de catalogue	HV514010
Description	InVivoMAb Anti-Human RSPO3 Antibody (Iv0181) [Iv0181] (HV514010) is a human monoclonal antibody detecting RSPO3 in ELISA, FCM, Neutralization. Suitable for Human. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Reactivité des espèces	Human
Applications	ELISA, FCM, Neutralization
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	Iv0181
Cible	RSPO3, hPWTSR, hRspo3, PWTSR, R-spondin-3, Thrombospondin type-1 domain-containing protein 2, Protein with TSP type-1 repeat, Roof plate-specific spondin-3, THSD2
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Produits associés	pH 7.4 Dilution Buffer
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	Q9BXY4
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte	R-spondin-3 (RSPO3) is a ~30 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. 1. de Lau, W. et al. (2011) Nature 476, 293-7. PMID: 21727895 2. Glinka, A. et al. (2011) EMBO reports 12, 1055-61. PMID: 21909076 3. Gong, X. et al. (2012) PloS one 7, e37137. PMID: 22615920 4. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Flow Cytometry

Flow-cytometry using anti-human RSPO3 antibody.RSPO3 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human RSPO3 antibody monoclonal antibody (Catalog # HV514010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human RSPO3 Antibody (Iv0181).