InVivoMAb Anti-Human TSG101 Antibody (Iv0253) (HV181010)

Name: InVivoMAb Anti-Human TSG101 Antibody (Iv0253)
Brand: abinScience
SKU: HV181010
Price: 370 USD
Availability: InStock

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Precio(USD):

Especificación:

100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV181010

Descripción

InVivoMAb Anti-Human TSG101 Antibody (Iv0253) [Iv0253] (HV181010) is a human monoclonal antibody detecting Tumor susceptibility gene 101 protein in ELISA, Neutralization. Suitable for Human.

Reactividad de especies

Human

Aplicaciones

ELISA, WB, FCM, Neutralization

Especie huésped

Human

Clonalidad

Monoclonal

Isotipo

IgG

ID de clon

Iv0253

Objetivo

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Productos asociados

pH 7.4 Dilution Buffer

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q99816

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation.

1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426

Nota

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Imágenes

Western blot

Western blot analysis was performed using anti-TSG101 monoclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: K562 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Lncap cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse brain lysate
Lane 8: Rat brain lysate
Lane 9: Rat heart lysate
Flow cytometry

Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.