InVivoMAb Anti-Human TSG101 Antibody (Iv0253) (HV181010)

Name: InVivoMAb Anti-Human TSG101 Antibody (Iv0253)
Brand: abinScience
SKU: HV181010
Price: 370 USD
Availability: InStock

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Prix(USD) :

Spécification :

100ug
1mg

Nombre :

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HV181010

Description

InVivoMAb Anti-Human TSG101 Antibody (Iv0253) [Iv0253] (HV181010) is a human monoclonal antibody detecting Tumor susceptibility gene 101 protein in ELISA, Neutralization. Suitable for Human.

Reactivité des espèces

Human

Applications

ELISA, WB, FCM, Neutralization

Espèce hôte

Human

Clonalité

Monoclonal

Isotype

IgG

ID de clone

Iv0253

Cible

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Produits associés

pH 7.4 Dilution Buffer

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q99816

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Tumor susceptibility gene 101 protein (TSG101) is a ~43 kDa protein. Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation.

1. Baietti, MF. et al. (2012) Nature cell biology 14, 677-85. PMID: 22660413

2. Nabhan, JF. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 4146-51. PMID: 22315426

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Western blot

Western blot analysis was performed using anti-TSG101 monoclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: K562 cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Lncap cell lysate
Lane 6: 293T cell lysate
Lane 7: Mouse brain lysate
Lane 8: Rat brain lysate
Lane 9: Rat heart lysate
Flow cytometry

Flow-cytometry using APC anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry using PE anti-human TSG101 antibody. Jurkat cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human TSG101 monoclonal antibody (Catalog HV181010, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.