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Mouse Anti-Human Kappa Chain Monoclonal Antibody, FITC (HB980415)

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Vista general
Número de catálogoHB980415
Descripción
Mouse Anti-Human Kappa Chain Monoclonal Antibody, FITC (HB980415) is a mouse monoclonal antibody detecting Kappa chain in ELISA, IF, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●FITC Conjugated — Ready for direct detection without secondary antibody.
Reactividad de especiesHuman
AplicacionesELISA, IF, WB
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG1
ConjugaciónFITC
Inmunógeno Nature Protein
Objetivo Second antibody, Second Ab, Mouse Anti-Human IgG, Kappa Chain Monoclonal Antibody, FITC
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Productos asociados Mouse IgG1 kappa Recombinant Isotype Control Antibody (HyHEL-10), FITC
Purificación Protein A/G purified from cell culture supernatant.
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento Store at 4°C 12 months.
Fondo

Immunoglobulin kappa constant (Kappa Chain) is a ~11 kDa protein. Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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