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Mouse Anti-Human Kappa Chain Monoclonal Antibody, FITC (HB980415)

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Aperçu
Numéro de catalogueHB980415
Description
Mouse Anti-Human Kappa Chain Monoclonal Antibody, FITC (HB980415) is a mouse monoclonal antibody detecting Kappa chain in ELISA, IF, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●FITC Conjugated — Ready for direct detection without secondary antibody.
Reactivité des espècesHuman
ApplicationsELISA, IF, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1
ConjugaisonFITC
Immunogène Nature Protein
Cible Second antibody, Second Ab, Mouse Anti-Human IgG, Kappa Chain Monoclonal Antibody, FITC
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Produits associés Mouse IgG1 kappa Recombinant Isotype Control Antibody (HyHEL-10), FITC
Purification Protein A/G purified from cell culture supernatant.
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C 12 months.
Contexte

Immunoglobulin kappa constant (Kappa Chain) is a ~11 kDa protein. Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens. The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain.

1. Schroeder, HW. et al. (2010) The Journal of allergy and clinical immunology 125, S41-52. PMID: 20176268
2. McHeyzer-Williams, M. et al. (2011) Nature reviews. Immunology 12, 24-34. PMID: 22158414
3. Teng, G. et al. (2007) Annual review of genetics 41, 107-20. PMID: 17576170
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Working Solution
C₂ (Working Conc.)
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