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PNGase F
PNGase F
PNGase F
PNGase F
PNGase F
PNGase F
Precio(USD):
Especificación:
  • 15KU
  • 75KU
Número:
Contáctenos
  • Vista general

  • Imágenes

  • Referencias

  • Datasheet

  • SDS

Vista general
Número de catálogoJN842012
Sistema de expresiónE. coli
Longitud de proteínaPNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.
AplicacionesRemoval of high mannose N-glycans from glycoproteins
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Forma Liquid
Buffer de almacenamiento 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosCAS: 83534-39-8
Referencias 1. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7. [JN842012]
2. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204. [JN842012]
Nota For research use only.
Imágenes
  • PNGase F

    SDS-PAGE

    SDS-PAGE for PNGase F

  • PNGase F

    Bioactivity

    Deglycosylation of Human IgG1 Fc by PNGase F.

  • PNGase F

    Bioactivity

    Schematic diagram of PNGase F cleavage site.

Referencias
Datasheet
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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