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| カタログ番号 | JN842012 |
|---|---|
| 発現システム | E. coli |
| タンパク質長 | PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli. |
| アプリケーション | Removal of high mannose N-glycans from glycoproteins |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >95% as determined by SDS-PAGE. |
| 形態 | Liquid |
| 保存バッファー | 20 mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | CAS: 83534-39-8 |
| 参考文献 | 1. Vilaj M, et al. Evaluation of different PNGase F enzymes in immunoglobulin G and total plasma N-glycans analysis. Glycobiology. 2021 Jan 9;31(1):2-7. [JN842012] 2. Huang J, et al. Highly Efficient Release of Glycopeptides from Hydrazide Beads by Hydroxylamine Assisted PNGase F Deglycosylation for N-Glycoproteome Analysis. Anal Chem. 2015 Oct 20;87(20):10199-204. [JN842012] |
| 注意事項 | For research use only. |
SDS-PAGE for PNGase F
Deglycosylation of Human IgG1 Fc by PNGase F.
Schematic diagram of PNGase F cleavage site.
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