Rat Anti-Human Glypican 3 Recombinant Antibody (YP7) (HW310053)

Name: Rat Anti-Human Glypican 3 Recombinant Antibody (YP7)
Brand: abinScience
SKU: HW310053
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HW310053

Reactividad de especies

Human

Aplicaciones

ELISA, FCM, WB

Especie huésped

Rat

Clonalidad

Monoclonal

Isotipo

IgG1, kappa

ID de clon

YP7

Aplicaciones probadas

WB, FCM

Objetivo

GPC3, MXR7, GTR2-2, Glypican-3, Intestinal protein OCI-5, OCI5

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

P51654

RRID

Rat Anti-Human GPC3 Antibody (YP7) (abinScience Cat# HW310053, RRID:AB_3729294)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

Glypican-3 (GPC3/Glypican 3) is a ~65 kDa protein. Cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway.

1. De Cat, B. et al. (2003) The Journal of cell biology 163, 625-35. PMID: 14610063

2. Capurro, MI. et al. (2005) The Journal of biological chemistry 280, 41201-6. PMID: 16227623

3. Capurro, M. et al. (2014) Journal of cell science 127, 1565-75. PMID: 24496449

4. Davoodi, J. et al. (2007) Proteomics 7, 2300-10. PMID: 17549790

Nota

For research use only.

Isotype Control

Rat IgG1, kappa Recombinant Isotype Control Antibody (RTK2071) (RV080407)

Imágenes

Bioactivity

Detects recombinant human Glypican-3/GPC3 (Cat No.: HW310011) in indirect ELISA.
Bioactivity

Detects recombinant mouse Glypican-3/GPC3 (Cat No.: MW310011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for Rat Anti-Human GPC3 Antibody (YP7).
Flow CytoMetry

Flow-cytometry using anti-human GPC3 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human GPC3 monoclonal antibody (Catalog: HW310053) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Rat IgG H&L Polyclonal Antibody, FITC (abinScience: ？) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-GPC3 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human GPC3 (Catalog No: HW310011)
Lane 2: negative control