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Arabidopsis thaliana KAI2 Recombinant Protein (N-His) (PR007012)

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概要
カタログ番号PR007012
説明
Recombinant Arabidopsis thaliana KAI2 Protein, N-His (PR007012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% as determined by SDS-PAGE.
発現システムE. coli
アクセッション番号Q9SZU7
タンパク質長Met1-Met270
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Arabidopsis thaliana (Mouse-ear cress)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名D14L, HTL, KAI2, Probable esterase KAI2, Protein D14-like, Protein DWARF-14-like, Protein HYPOSENSITIVE TO LIGHT, Protein KARRIKIN INSENSITIVE 2
背景

Probable esterase KAI2 is a ~29 kDa protein. Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Lacks detectable hydrolase activity against karrikin. Karrikin binding induces a conformational change.

1. Sun, XD. et al. (2011) Molecular plant 4, 116-26. PMID: 20864454
2. Waters, MT. et al. (2012) Development (Cambridge, England) 139, 1285-95. PMID: 22357928
3. Waters, MT. et al. (2013) Molecular plant 6, 63-75. PMID: 23142794
4. Kagiyama, M. et al. (2013) Genes to cells : devoted to molecular & cellular mechanisms 18, 147-60. PMID: 23301669
5. Guo, Y. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8284-9. PMID: 23613584
注意事項 For research use only
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参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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C₁ (Stock Conc.)
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Working Solution
C₂ (Working Conc.)
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