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Anti-Arabidopsis thaliana KAI2 Polyclonal Antibody (PR007014)

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Overview
Catalog No.PR007014
Description
Anti-Arabidopsis thaliana KAI2 Polyclonal Antibody (PR007014) is a rabbit polyclonal antibody detecting KAI2. Suitable for Arabidopsis thaliana.
Species reactivityArabidopsis thaliana (Mouse-ear cress)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Arabidopsis thaliana KAI2 (Met1-Met270).
Target D14L, HTL, KAI2, Probable esterase KAI2, Protein D14-like, Protein DWARF-14-like, Protein HYPOSENSITIVE TO LIGHT, Protein KARRIKIN INSENSITIVE 2
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q9SZU7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Probable esterase KAI2 is a ~29 kDa protein. Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Lacks detectable hydrolase activity against karrikin. Karrikin binding induces a conformational change.

1. Sun, XD. et al. (2011) Molecular plant 4, 116-26. PMID: 20864454
2. Waters, MT. et al. (2012) Development (Cambridge, England) 139, 1285-95. PMID: 22357928
3. Waters, MT. et al. (2013) Molecular plant 6, 63-75. PMID: 23142794
4. Kagiyama, M. et al. (2013) Genes to cells : devoted to molecular & cellular mechanisms 18, 147-60. PMID: 23301669
5. Guo, Y. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8284-9. PMID: 23613584
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Arabidopsis thaliana KAI2 Polyclonal Antibody

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