

| Catalog No. | PR007012 |
|---|---|
| Description |
Recombinant Arabidopsis thaliana KAI2 Protein, N-His (PR007012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| Expression system | E. coli |
| Accession | Q9SZU7 |
| Protein length | Met1-Met270 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Arabidopsis thaliana (Mouse-ear cress) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | D14L, HTL, KAI2, Probable esterase KAI2, Protein D14-like, Protein DWARF-14-like, Protein HYPOSENSITIVE TO LIGHT, Protein KARRIKIN INSENSITIVE 2 |
| Background | Probable esterase KAI2 is a ~29 kDa protein. Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Lacks detectable hydrolase activity against karrikin. Karrikin binding induces a conformational change. 1. Sun, XD. et al. (2011) Molecular plant 4, 116-26. PMID: 20864454 2. Waters, MT. et al. (2012) Development (Cambridge, England) 139, 1285-95. PMID: 22357928 3. Waters, MT. et al. (2013) Molecular plant 6, 63-75. PMID: 23142794 4. Kagiyama, M. et al. (2013) Genes to cells : devoted to molecular & cellular mechanisms 18, 147-60. PMID: 23301669 5. Guo, Y. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8284-9. PMID: 23613584 |
| Note | For research use only |
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