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Arabidopsis thaliana KAI2 Recombinant Protein (N-His) (PR007012)

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Overview
Catalog No.PR007012
Description
Recombinant Arabidopsis thaliana KAI2 Protein, N-His (PR007012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ9SZU7
Protein lengthMet1-Met270
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesArabidopsis thaliana (Mouse-ear cress)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesD14L, HTL, KAI2, Probable esterase KAI2, Protein D14-like, Protein DWARF-14-like, Protein HYPOSENSITIVE TO LIGHT, Protein KARRIKIN INSENSITIVE 2
Background

Probable esterase KAI2 is a ~29 kDa protein. Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones. Lacks detectable hydrolase activity against karrikin. Karrikin binding induces a conformational change.

1. Sun, XD. et al. (2011) Molecular plant 4, 116-26. PMID: 20864454
2. Waters, MT. et al. (2012) Development (Cambridge, England) 139, 1285-95. PMID: 22357928
3. Waters, MT. et al. (2013) Molecular plant 6, 63-75. PMID: 23142794
4. Kagiyama, M. et al. (2013) Genes to cells : devoted to molecular & cellular mechanisms 18, 147-60. PMID: 23301669
5. Guo, Y. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8284-9. PMID: 23613584
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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