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ASFV D117L Recombinant Protein (N-His-KSI) (VK023012)

ASFV D117L Recombinant Protein (N-His-KSI)
ASFV D117L Recombinant Protein (N-His-KSI)
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Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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Vista general
Número de catálogoVK023012
Descripción
Recombinant ASFV D117L Protein, N-His-KSI (VK023012) expressed in E. coli. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoQ89424
Longitud de proteínaAsp2-Thr38&Tyr60-Ser117
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesAfrican swine fever virus (ASFV)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 26.65 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosMinor capsid protein p17
Fondo

Minor capsid protein p17 is a ~13 kDa protein. Together with the penton and the other minor capsid proteins (M1249L, p49), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. Three copies of p17 encircle each p72 capsomer in the inner capsid shell, anchoring p72 capsomers on the inner membrane. Required for the assembly of the capsid and icosahedral morphogenesis. Additionally, inhibits the host cGAS-STING pathway through its interaction with STING1 and subsequent interference of the recruitment of downstream components TBK1 and IKBKE.

1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094
2. Suárez, C. et al. (2010) Journal of virology 84, 7484-99. PMID: 20504920
3. Zheng, W. et al. (2022) Frontiers in immunology 13, 941579. PMID: 35844609
Nota For research use only
Imágenes
  • ASFV D117L Recombinant Protein (N-His-KSI)

    SDS-PAGE

    SDS-PAGE for Recombinant ASFV D117L Protein, N-His-KSI

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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