Anti-ASFV D117L Polyclonal Antibody (VK023014)

Name: Anti-ASFV D117L Polyclonal Antibody
Brand: abinScience
SKU: VK023014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

VK023014

Description

Anti-ASFV D117L Polyclonal Antibody (VK023014) is a rabbit polyclonal antibody detecting ASFV D117L in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

African swine fever virus (ASFV)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV D117L (Asp2-Thr38&Tyr60-Ser117).

Target

Minor capsid protein p17

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q89424

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
WB	1:500-1:2000
IHC	1:50-1:500

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Minor capsid protein p17 is a ~13 kDa protein. Together with the penton and the other minor capsid proteins (M1249L, p49), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. Three copies of p17 encircle each p72 capsomer in the inner capsid shell, anchoring p72 capsomers on the inner membrane. Required for the assembly of the capsid and icosahedral morphogenesis. Additionally, inhibits the host cGAS-STING pathway through its interaction with STING1 and subsequent interference of the recruitment of downstream components TBK1 and IKBKE.

1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094

2. Suárez, C. et al. (2010) Journal of virology 84, 7484-99. PMID: 20504920

3. Zheng, W. et al. (2022) Frontiers in immunology 13, 941579. PMID: 35844609

Note

For research use only

Images

Western blot

Lane 1: recombinant ASFV D117L (Catalog No: VK023012)

References

Datasheet

Anti-ASFV D117L Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ASFV D117L Polyclonal Antibody (VK023014)

Overview

Images

References

Datasheet

SDS

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