ASFV pM1249L Recombinant Protein (N-His) (VK776012)

Name: ASFV pM1249L Recombinant Protein (N-His)
Brand: abinScience
SKU: VK776012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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SDS

Обзор

Каталожный номер	VK776012
Описание	Recombinant ASFV pM1249L Protein, N-His (VK776012) expressed in E. coli, spanning Gln346-Pro452. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q65152
Длина белка	Gln346-Pro452
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	African swine fever virus (ASFV)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	14.57 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Minor capsid protein M1249L, pM1249L, Ba71V-060, M1249L
Фон	Minor capsid protein M1249L (pM1249L) is a ~144 kDa protein. Together with the penton and the other minor capsid proteins (p17, p49), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. In addition, blocks IFN-beta transactivation mediated by the cGAS-STING pathway and regulates the transcriptional activity of IFN-beta. Mechanistically, suppresses the phosphorylation of host key adapter protein TBK1 and degrades host IRF3 in the cytoplasm. 1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094 2. Cui, S. et al. (2022) Virus research 319, 198872. PMID: 35853521
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant ASFV pM1249L Protein, N-His

Ссылки

Даташит

ASFV pM1249L Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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