ASFV S273R Recombinant Protein (N-His) (VK573012)

Name: ASFV S273R Recombinant Protein (N-His)
Brand: abinScience
SKU: VK573012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	VK573012
Description	Recombinant ASFV S273R Protein, N-His (VK573012) expressed in E. coli, spanning Met1-Ala273. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q00946
Longueur de protéine	Met1-Ala273
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	African swine fever virus (ASFV)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	33.71 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Cysteine protease S273R, pS273R, 3.4.22.-, Ba71V-111, S273R
Contexte	Cysteine protease S273R is a ~31 kDa protein. Cysteine protease that plays several role during infection including processing of the structural polyprotein or inhibition of the host immune response. Catalyzes the maturation of the pp220 and pp62 polyprotein precursors into core-shell proteins. Plays a role in the disruption of host pyroptosis via specific cleavage of gasdermin D/GSDMD. In addition, strongly decreases the host cGAS-STING signaling by targeting IKBKE via its enzymatic activity. Also impairs host FOXJ1-mediated antiviral effect via degradation of FOXJ1. 1. Andrés, G. et al. (2002) Journal of virology 76, 12473-82. PMID: 12438573 2. Zhao, G. et al. (2022) The Journal of biological chemistry 298, 101480. PMID: 34890644 3. Luo, J. et al. (2022) Virulence 13, 740-756. PMID: 35437104 4. Ma, C. et al. (2022) Virologica Sinica 37, 445-454. PMID: 35513267 5. Li, T. et al. (2023) The Journal of biological chemistry 299, 104844. PMID: 37209818 6. Li, YH. et al. (2023) Journal of virology 97, e0194222. PMID: 36856422
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant ASFV S273R Protein, N-His

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ASFV S273R Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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