Anti-ASFV S273R Polyclonal Antibody (VK573014)

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Overview

Catalog No.

VK573014

Description

Anti-ASFV S273R Polyclonal Antibody (VK573014) is a rabbit polyclonal antibody detecting S273R in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

African swine fever virus

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant ASFV S273R (Met1-Ala273).

Target

Cysteine protease S273R, pS273R, 3.4.22.-, Ba71V-111, S273R

Purification

Purified by antigen affinity column.

Accession

Q00946

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cysteine protease S273R is a ~31 kDa protein. Cysteine protease that plays several role during infection including processing of the structural polyprotein or inhibition of the host immune response. Catalyzes the maturation of the pp220 and pp62 polyprotein precursors into core-shell proteins. Plays a role in the disruption of host pyroptosis via specific cleavage of gasdermin D/GSDMD. In addition, strongly decreases the host cGAS-STING signaling by targeting IKBKE via its enzymatic activity. Also impairs host FOXJ1-mediated antiviral effect via degradation of FOXJ1.

1. Andrés, G. et al. (2002) Journal of virology 76, 12473-82. PMID: 12438573

2. Zhao, G. et al. (2022) The Journal of biological chemistry 298, 101480. PMID: 34890644

3. Luo, J. et al. (2022) Virulence 13, 740-756. PMID: 35437104

4. Ma, C. et al. (2022) Virologica Sinica 37, 445-454. PMID: 35513267

5. Li, T. et al. (2023) The Journal of biological chemistry 299, 104844. PMID: 37209818

6. Li, YH. et al. (2023) Journal of virology 97, e0194222. PMID: 36856422

Note

For research use only.

Images

References

Datasheet

Anti-ASFV S273R Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ASFV S273R Polyclonal Antibody (VK573014)

Overview

Images

References

Datasheet

SDS

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