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| カタログ番号 | JN161022 |
|---|---|
| 説明 |
Recombinant C. botulinum botE Protein, N-His (JN161022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q00496 |
| タンパク質長 | Ser850-Lys1251 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Clostridium botulinum |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | BoNT/E, Bontoxilysin-E, Botulinum neurotoxin E heavy chain, Botulinum neurotoxin E light chain, Botulinum neurotoxin type E, EC:3.4.24.69, HC, LC, botE |
| 背景 | Botulinum neurotoxin type E is a ~143 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin E which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol. 1. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346 2. Rummel, A. et al. (2009) Journal of neurochemistry 110, 1942-54. PMID: 19650874 3. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883 4. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274 |
| 注意事項 | For research use only |
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