Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610) (JN161013)

Name: Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610)
Brand: abinScience
SKU: JN161013
Price: 191 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

JN161013

Species reactivity

Clostridium botulinum

Applications

ELISA

Host species

Mouse

Isotype

IgG

Clone ID

SAA2610

Clonality

Monoclonal

Target

BoNT/E, Bontoxilysin-E, Botulinum neurotoxin E heavy chain, Botulinum neurotoxin E light chain, Botulinum neurotoxin type E, EC:3.4.24.69, HC, LC, botE

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q00496

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Botulinum neurotoxin type E (BoNT) is a ~143 kDa protein. Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure. Precursor of botulinum neurotoxin E which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins found in synaptic vesicles. Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them. Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway. When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol.

1. Fu, Z. et al. (2009) Biochemistry 48, 5631-41. PMID: 19476346

2. Rummel, A. et al. (2009) Journal of neurochemistry 110, 1942-54. PMID: 19650874

3. Sun, S. et al. (2012) Biochemistry 51, 5655-62. PMID: 22720883

4. Dong, M. et al. (2008) Molecular biology of the cell 19, 5226-37. PMID: 18815274

Note

For research use only

Images

References

Datasheet

Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Clostridium botulinum BoNT/E Recombinant Antibody (SAA2610) (JN161013)

Overview

Images

References

Datasheet

SDS

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