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| カタログ番号 | JN963012 |
|---|---|
| 説明 |
Recombinant Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Protein, N-His (JN963012) expressed in E. coli, spanning Asp30-Asn500. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P0C2E9 |
| タンパク質長 | Asp30-Asn500 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Clostridium perfringens |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 54.87 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163 |
| 背景 | Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation. |
| 注意事項 | For research use only. |
SDS-PAGE for Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Recombinant Protein (N-His)
Detects HS1 in indirect ELISAs.
Detected by Anti-Theta-toxin monoclonal antibody (Catalog No: JN963033)
Detected by Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS2) (Catalog No: JN963013) in indirect ELISA
Detected by Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS3) (Catalog No: JN963023) in indirect ELISA
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