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DENV-2 Envelope protein E Recombinant Protein (N-His) (VK589032)

DENV-2 Envelope protein E Recombinant Protein (N-His)
DENV-2 Envelope protein E Recombinant Protein (N-His)
DENV-2 Envelope protein E Recombinant Protein (N-His)
DENV-2 Envelope protein E Recombinant Protein (N-His)
DENV-2 Envelope protein E Recombinant Protein (N-His)
DENV-2 Envelope protein E Recombinant Protein (N-His)
Precio(USD):
Especificación:
  • 50ug
  • 100ug
  • 1mg
Número:
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  • Imágenes

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  • Datasheet

  • SDS

Vista general
Número de catálogoVK589032
Descripción
Recombinant DENV-2 Envelope protein E Protein, N-His (VK589032) expressed in E. coli, spanning Met477-Gln580. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Longitud de proteínaMet477-Gln580
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesDengue virus 2 (DENV-2)
Naturaleza Recombinant
Objetivo Envelope protein E
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 13.73 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosEnvelope protein E
Fondo

Genome polyprotein (Envelope protein E) is a ~74 kDa protein. Prevents premature fusion activity of envelope proteins in trans-Golgi by binding to envelope protein E at pH6.0. After virion release in extracellular space, gets dissociated from E dimers.

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Nota For research use only.
Imágenes
  • DENV-2 Envelope protein E Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant DENV-2 Envelope protein E Protein, N-His

  • DENV-2 Envelope protein E Recombinant Protein (N-His)

    Bioactivity

    Detected by Iv0124 (Catalog No: VK589010) in indirect ELISA

  • DENV-2 Envelope protein E Recombinant Protein (N-His)

    Bioactivity

    Detected by 2C8 (Catalog No: VK589030) in indirect ELISA

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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