E. coli shiga toxin type 1 B/stx1B Recombinant Protein (N-His-SUMO) (JN072012)

Name: E. coli shiga toxin type 1 B/stx1B Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: JN072012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	JN072012
Описание	Recombinant E. coli shiga toxin type 1 B/stx1B Protein, N-His-SUMO (JN072012) expressed in E. coli, spanning Thr21-Arg89. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q7BQ98, Q32GM0, P69178, P69179, Q779K3
Длина белка	Thr21-Arg89
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Escherichia coli
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	20.03 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Shiga toxin 1 B subunit, Shiga toxin 1 subunit B, stx1, stx1B, ECs_2973
Фон	Shiga toxin subunit B (shiga toxin type 1 B/stx1B) is a ~9 kDa protein. The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
Примечание	For research use only.

Изображения

Western blot

Detected by Anti-coli monoclonal antibody (Catalog No: JN072013)
Bioactivity

Detected by Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) (Catalog No: JN072013) in indirect ELISA