Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) (JN072013)

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Datasheet

Overview

Catalog No.

JN072013

Description

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) [5-5B] (JN072013) is a mouse monoclonal antibody detecting Escherichia coli O157:H7 in ELISA, FCM, WB.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Escherichia coli O157:H7

Applications

ELISA, FCM, WB

Host species

Mouse

Isotype

IgG2a

Clone ID

5-5B

Clonality

Monoclonal

Target

stx1, stx1B, Shiga toxin 1 B subunit, Shiga toxin I B subunit, Escherichia coli O157:H7

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q7BQ98, Q32GM0, P69178, P69179, Q779K3

RRID

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) (abinScience Cat# JN072013, RRID:AB_3729026)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Shiga toxin subunit B (shiga toxin type 1B/stx1B) is a ~9 kDa protein. The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.

Note

For research use only.

Images

Bioactivity

SEC-HPLC detection for Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B).
Western blot

Western blot analysis was performed using anti-stx1B monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant E. coli stx1B (Catalog No: JN072012)
Lane 2: negative control
Bioactivity

Detects recombinant E. coli shiga toxin type 1 B/stx1B (Catalog No: JN072012) in indirect ELISA

References

Datasheet

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B).pdf

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Escherichia coli O157:H7 shiga toxin type 1 B/stx1B Antibody (5-5B) (JN072013)

Overview

Images

References

Datasheet

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