EBV/HHV4 BMRF1 Recombinant Protein (N-His) (VK074012)

Name: EBV/HHV4 BMRF1 Recombinant Protein (N-His)
Brand: abinScience
SKU: VK074012
Price: 211 USD
Availability: InStock

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1mg

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Vista general

Número de catálogo	VK074012
Descripción	Recombinant EBV/HHV4 BMRF1 Protein, N-His (VK074012) expressed in E. coli, spanning Met1-Ser314. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P03191
Longitud de proteína	Met1-Ser314
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Epstein-Barr virus (HHV-4) (Human herpesvirus 4)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	35.64 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	DNA polymerase processivity factor BMRF1, Early Antigen Diffused, EA-D, Polymerase accessory subunit
Fondo	DNA polymerase processivity factor BMRF1 is a ~43 kDa protein. Acts as a DNA polymerase processivity factor; a transcriptional activator for several EBV promoters and inhibits the host DNA damage response (DDR) to double-stranded DNA breaks. Plays an essential role in the viral lytic DNA replication by acting as a polymerase accessory subunit. Stimulates the viral DNA polymerase activity and appears to function with it as a holoenzyme. Increases the processivity of the viral polymerase, probably by acting as a sliding clamp that prevents dissociation of the polymerase from the active template. In addition, BMRF1 transcriptionally activates the oriLyt early BHLF1 promoter. 1. Neuhierl, B. et al. (2006) Journal of virology 80, 5078-81. PMID: 16641300 2. Murayama, K. et al. (2009) The Journal of biological chemistry 284, 35896-905. PMID: 19801550 3. Salamun, SG. et al. (2019) Journal of virology 93. PMID: 31462557 4. Zhang, Q. et al. (1996) Journal of virology 70, 5131-42. PMID: 8764021 5. Zhang, Q. et al. (1997) Virology 230, 22-34. PMID: 9126259
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant EBV/HHV4 BMRF1 Protein, N-His

Referencias

Datasheet

EBV/HHV4 BMRF1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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