Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014)

Name: Anti-EBV/HHV4 BMRF1 Polyclonal Antibody
Brand: abinScience
SKU: VK074014
Price: 168 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

VK074014

Description

Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014) is a rabbit polyclonal antibody detecting DNA polymerase processivity factor BMRF1 in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Epstein-Barr virus (HHV-4) (Human herpesvirus 4)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant EBV/HHV4 BMRF1 (Met1-Ser314).

Target

DNA polymerase processivity factor BMRF1, Early Antigen Diffused, EA-D, Polymerase accessory subunit

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P03191

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase processivity factor BMRF1 is a ~43 kDa protein. Acts as a DNA polymerase processivity factor; a transcriptional activator for several EBV promoters and inhibits the host DNA damage response (DDR) to double-stranded DNA breaks. Plays an essential role in the viral lytic DNA replication by acting as a polymerase accessory subunit. Stimulates the viral DNA polymerase activity and appears to function with it as a holoenzyme. Increases the processivity of the viral polymerase, probably by acting as a sliding clamp that prevents dissociation of the polymerase from the active template. In addition, BMRF1 transcriptionally activates the oriLyt early BHLF1 promoter.

1. Neuhierl, B. et al. (2006) Journal of virology 80, 5078-81. PMID: 16641300

2. Murayama, K. et al. (2009) The Journal of biological chemistry 284, 35896-905. PMID: 19801550

3. Salamun, SG. et al. (2019) Journal of virology 93. PMID: 31462557

4. Zhang, Q. et al. (1996) Journal of virology 70, 5131-42. PMID: 8764021

5. Zhang, Q. et al. (1997) Virology 230, 22-34. PMID: 9126259

Note

For research use only

Images

References

Datasheet

Anti-EBV/HHV4 BMRF1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-EBV/HHV4 BMRF1 Polyclonal Antibody (VK074014)

Overview

Images

References

Datasheet

SDS

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