HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His) (VK420012)

Name: HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: VK420012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	VK420012
Описание	Recombinant HMPV M2/Protein M2-2 Protein, N-GST & C-His (VK420012) expressed in E. coli, spanning Thr2-Ser71. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q6WB96
Длина белка	Thr2-Ser71
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Human metapneumovirus (HMPV)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	36.24 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Protein M2-2, M2
Фон	Protein M2-2 is a ~8 kDa protein. Mediates the regulatory switch from transcription to RNA replication. Acts late in infection by inhibiting viral transcription and up-regulating RNA replication. Plays a major role in antagonizing the type I IFN-mediated antiviral response. Interacts with host MAVS and prevents the interaction with its upstream partner RIGI in the signaling pathway leading to interferon production. Inhibits host RIGI signaling by preventing TRIM25-mediated RIGI ubiquitination. 1. Ren, J. et al. (2012) Journal of virology 86, 13049-61. PMID: 23015697 2. Tanaka, Y. et al. (2022) Frontiers in immunology 13, 970750. PMID: 36045682 3. Ren, J. et al. (2014) PloS one 9, e91865. PMID: 24618691 4. Kitagawa, Y. et al. (2017) Journal of virology 91. PMID: 28768858
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant HMPV M2/Protein M2-2 Protein, N-GST & C-His

Ссылки

Даташит

HMPV M2/Protein M2-2 Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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