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HRSV G/Major surface glycoprotein G Recombinant Protein (C-His) (VK562011)

HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)
HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)
HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)
HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)
HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)
HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)
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Aperçu
Numéro de catalogueVK562011
Description
Recombinant HRSV G/Major surface glycoprotein G Protein, C-His (VK562011) expressed in Mammalian Cells, spanning Asn66-Gln298. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●Mammalian Expression — Proper folding and native glycosylation.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionMammalian cells
Numéro d'accèsP20895
Longueur de protéineAsn66-Gln298
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHuman respiratory syncytial virus A (HRSV-A)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 28.35 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsMajor surface glycoprotein G, Attachment glycoprotein G, Membrane-bound glycoprotein, mG, Mature secreted glycoprotein G, Mature sG, G
Contexte

Major surface glycoprotein G is a ~32 kDa protein. Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities.

Note For research use only.
Images
  • HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)

    Bioactivity

    Detects HRSV G/Major surface glycoprotein G Antibody (CB002.5) in indirect ELISAs.

  • HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)

    Bioactivity

    Detected by Anti-HRSV-A/B G/Major surface glycoprotein G Antibody (131-2G) (Cat No.: VK562033) in indirect ELISA.

  • HRSV G/Major surface glycoprotein G Recombinant Protein (C-His)

    Bioactivity

    Detected by InVivoMAb Anti-HRSV G/Major surface glycoprotein G Antibody (3G12) (Catalog No: VK562030) in indirect ELISA

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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