Human ABCA3 Recombinant Protein (N-His) (HV604012)

Name: Human ABCA3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV604012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HV604012
Descripción	Recombinant Human ABCA3 Protein, N-His (HV604012) expressed in E. coli, spanning Asp1358-Phe1635. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q99758
Longitud de proteína	Asp1358-Phe1635
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	32.85 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	ABCA3, ATP-binding cassette sub-family A member 3, Phospholipid-transporting ATPase ABCA3, ABC3, ATP-binding cassette 3, ATP-binding cassette transporter 3, ABC-C transporter, Xenobiotic-transporting ATPase ABCA3
Fondo	Phospholipid-transporting ATPase ABCA3 is a ~191 kDa protein. Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. 1. Matsumura, Y. et al. (2006) The Journal of biological chemistry 281, 34503-14. PMID: 16959783 2. Matsumura, Y. et al. (2007) FEBS letters 581, 3139-44. PMID: 17574245 3. Wittmann, T. et al. (2016) Pediatric pulmonology 51, 1284-1294. PMID: 27177387 4. Höppner, S. et al. (2017) Biochimica et biophysica acta. Molecular cell research 1864, 2330-2335. PMID: 28887056 5. Li, Y. et al. (2019) Biochimica et biophysica acta. Molecular and cell biology of lipids 1864, 158516. PMID: 31473345 6. Zarbock, R. et al. (2015) Biochimica et biophysica acta 1851, 987-95. PMID: 25817392 7. Dohmen, LC. et al. (2016) The Journal of biological chemistry 291, 9638-47. PMID: 26903515
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human ABCA3 Protein, N-His

Referencias

Datasheet

Human ABCA3 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

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Volume

Molecular Weight *

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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