Human ACADSB Recombinant Protein (N-His) (HW543012)

Name: Human ACADSB Recombinant Protein (N-His)
Brand: abinScience
SKU: HW543012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HW543012
Description	Recombinant Human ACADSB Protein, N-His (HW543012) expressed in E. coli, spanning Thr58-Tyr432. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P45954
Longueur de protéine	Thr58-Tyr432
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	43.38 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	2-methyl branched chain acyl-CoA dehydrogenase, SBCAD, 2-MEBCAD, 2-methylbutyryl-CoA dehydrogenase, ACADSB, Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial, 2-methylbutyryl-coenzyme A dehydrogenase
Contexte	Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial (ACADSB) is a ~47 kDa protein. Short and branched chain specific acyl-CoA dehydrogenase that catalyzes the proR-proR stereospecific alpha,beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). Among the different mitochondrial acyl-CoA dehydrogenases, acts specifically on short and branched chain acyl-CoA derivatives such as (S)-2-methylbutyryl-CoA as well as short straight chain acyl-CoAs such as butyryl-CoA (butanoyl-CoA). Plays an important role in the metabolism of L-isoleucine by catalyzing the dehydrogenation of 2-methylbutyryl-CoA, one of the steps of the L-isoleucine catabolic pathway. Can also act on valproyl-CoA, a metabolite of valproic acid, an antiepileptic drug. 1. Gibson, KM. et al. (2000) Pediatric research 47, 830-3. PMID: 10832746 2. Andresen, BS. et al. (2000) American journal of human genetics 67, 1095-103. PMID: 11013134 3. Luís, PB. et al. (2011) Drug metabolism and disposition: the biological fate of chemicals 39, 1155-60. PMID: 21430231 4. Rozen, R. et al. (1994) Genomics 24, 280-7. PMID: 7698750
Note	For research use only.

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SDS-PAGE for Recombinant Human ACADSB Protein, N-His

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Human ACADSB Recombinant Protein (N-His).pdf

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