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Human ACADVL Recombinant Protein (N-His) (HC227012)

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Vista general
Número de catálogoHC227012
Descripción
Recombinant Human ACADVL Protein, N-His (HC227012) expressed in E. coli, spanning Glu96-Phe655. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónE. coli
Número de accesoP49748
Longitud de proteínaGlu96-Phe655
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 62.71 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosACADVL, EC:1.3.8.9, VLCAD, Very long-chain specific acyl-CoA dehydrogenase, mitochondrial
Fondo

Very long-chain acyl-CoA dehydrogenase, mitochondrial is a ~70 kDa protein. Very long-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation (FAO), breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of FAO consists in the proR-proR stereospecific alpha, beta-dehydrogenation of fatty acyl-CoA thioesters using the electron transfer flavoprotein (ETF) as their physiologic electron acceptor, resulting in the formation of trans-2-enoyl-CoA ((2E)-enoyl-CoA). Among the different mitochondrial acyl-CoA dehydrogenases, very long-chain specific acyl-CoA dehydrogenase acts specifically on fatty acyl-CoAs with saturated 12 to 24 carbons long primary chains.

1. He, M. et al. (2007) American journal of human genetics 81, 87-103. PMID: 17564966
2. McAndrew, RP. et al. (2008) The Journal of biological chemistry 283, 9435-43. PMID: 18227065
3. Aoyama, T. et al. (1995) American journal of human genetics 57, 273-83. PMID: 7668252
4. Souri, M. et al. (1998) The Journal of biological chemistry 273, 4227-31. PMID: 9461620
5. Souri, M. et al. (1998) FEBS letters 426, 187-90. PMID: 9599005
6. Souri, M. et al. (1998) European journal of biochemistry 257, 592-8. PMID: 9839948
7. He, M. et al. (2011) Molecular genetics and metabolism 102, 418-29. PMID: 21237683
Nota For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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