

| Número de catálogo | HT030012 |
|---|---|
| Descripción |
Recombinant Human ACOT7 Protein, N-His (HT030012) expressed in E. coli, spanning Gly52-Pro380. Purity: >90% by SDS-PAGE.
Highlights
|
| Sistema de expresión | E. coli |
| Número de acceso | O00154 |
| Longitud de proteína | Gly52-Pro380 |
| Aplicaciones | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Especies | Homo sapiens (Human) |
| Naturaleza | Recombinant |
| Nivel de endotoxinas | Please contact with the lab for this information. |
| Pureza | >90% as determined by SDS-PAGE. |
| Peso molecular predicho | 38.69 kDa |
| Forma | Lyophilized |
| Buffer de almacenamiento | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Reconstitución | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Envío | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Nombres alternativos | Cytosolic acyl coenzyme A thioester hydrolase, 3.1.2.2, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, BACH, hBACH, CTE-IIa, CTE-II, Long chain acyl-CoA thioester hydrolase, ACOT7, BACH |
| Fondo | Cytosolic acyl coenzyme A thioester hydrolase (ACOT7) is a ~41 kDa protein. Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18. May play an important physiological function in brain. 1. Yamada, J. et al. (1999) Journal of biochemistry 126, 1013-9. PMID: 10578051 |
| Nota | For research use only. |

SDS-PAGE for Recombinant Human ACOT7 Protein, N-His
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