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Anti-Human ACOT7 Polyclonal Antibody (HT030014)

Anti-Human ACOT7 Polyclonal Antibody
Anti-Human ACOT7 Polyclonal Antibody
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Overview
Catalog No.HT030014
Description
Anti-Human ACOT7 Polyclonal Antibody (HT030014) is a rabbit polyclonal antibody detecting ACOT7 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ACOT7 (Gly52-Pro380).
Target Cytosolic acyl coenzyme A thioester hydrolase, 3.1.2.2, Acyl-CoA thioesterase 7, Brain acyl-CoA hydrolase, BACH, hBACH, CTE-IIa, CTE-II, Long chain acyl-CoA thioester hydrolase, ACOT7, BACH
Purification Purified by antigen affinity column.
Accession O00154
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Cytosolic acyl coenzyme A thioester hydrolase (ACOT7) is a ~41 kDa protein. Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Preferentially hydrolyzes palmitoyl-CoA, but has a broad specificity acting on other fatty acyl-CoAs with chain-lengths of C8-C18. May play an important physiological function in brain.

1. Yamada, J. et al. (1999) Journal of biochemistry 126, 1013-9. PMID: 10578051
Note For research use only.
Images
  • Anti-Human ACOT7 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ACOT7 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Rat brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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