Human ADA/Adenosine deaminase Recombinant Protein (N-His-SUMO) (HF562022)

Name: Human ADA/Adenosine deaminase Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HF562022
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HF562022
Descripción	Recombinant Human ADA/Adenosine deaminase Protein, N-His-SUMO (HF562022) expressed in E. coli, spanning Thr57-Leu363. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P00813
Longitud de proteína	Thr57-Leu363
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	46.97 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Adenosine aminohydrolase, Adenosine deaminase, ADA1, ADA
Fondo	Adenosine deaminase (ADA) is a ~40 kDa protein. Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. 1. Eltzschig, HK. et al. (2006) Blood 108, 1602-10. PMID: 16670267 2. Gracia, E. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1048-61. PMID: 23193172 3. Fattahi, A. et al. (2015) Urology 86, 730-4. PMID: 26166670 4. Lindley, ER. et al. (1993) The Biochemical journal 290 ( Pt 2), 457-62. PMID: 8452534 5. Jiang, C. et al. (1997) Human molecular genetics 6, 2271-8. PMID: 9361033 6. Martinez-Navio, JM. et al. (2011) Journal of leukocyte biology 89, 127-36. PMID: 20959412 7. Ginés, S. et al. (2002) The Biochemical journal 361, 203-9. PMID: 11772392 8. Gonzalez-Gronow, M. et al. (2004) The Journal of biological chemistry 279, 20993-8. PMID: 15016824 10. Li, G. et al. (2015) Pharmacology research & perspectives 3, e00121. PMID: 26038697
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human ADA/Adenosine deaminase Protein, N-His-SUMO

Referencias

Datasheet

Human ADA/Adenosine deaminase Recombinant Protein (N-His-SUMO).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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