

| Catalog No. | HF562032 |
|---|---|
| Description |
Recombinant Human ADA Protein, N-His (HF562032) expressed in E. coli, spanning Thr57-Leu363. Purity: >90% by SDS-PAGE.
Highlights
|
| Expression system | E. coli |
| Accession | P00813 |
| Protein length | Thr57-Leu363 |
| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Species | Homo sapiens (Human) |
| Nature | Recombinant |
| Endotoxin level | Please contact with the lab for this information. |
| Purity | >90% as determined by SDS-PAGE. |
| Predicted molecular weight | 36.92 kDa |
| Form | Lyophilized |
| Storage buffer | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Reconstitution | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Shipping | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | ADA, ADA1, Adenosine aminohydrolase, Adenosine deaminase, EC:3.5.4.4 |
| Background | Adenosine deaminase is a ~40 kDa protein. Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. 1. Eltzschig, HK. et al. (2006) Blood 108, 1602-10. PMID: 16670267 2. Gracia, E. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1048-61. PMID: 23193172 3. Fattahi, A. et al. (2015) Urology 86, 730-4. PMID: 26166670 4. Lindley, ER. et al. (1993) The Biochemical journal 290 ( Pt 2), 457-62. PMID: 8452534 5. Jiang, C. et al. (1997) Human molecular genetics 6, 2271-8. PMID: 9361033 6. Martinez-Navio, JM. et al. (2011) Journal of leukocyte biology 89, 127-36. PMID: 20959412 7. Ginés, S. et al. (2002) The Biochemical journal 361, 203-9. PMID: 11772392 8. Gonzalez-Gronow, M. et al. (2004) The Journal of biological chemistry 279, 20993-8. PMID: 15016824 10. Li, G. et al. (2015) Pharmacology research & perspectives 3, e00121. PMID: 26038697 |
| Note | For research use only |


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