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Human ADA Recombinant Protein (N-His) (HF562032)

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Overview
Catalog No.HF562032
Description
Recombinant Human ADA Protein, N-His (HF562032) expressed in E. coli, spanning Thr57-Leu363. Purity: >90% by SDS-PAGE.
Highlights
  • His-Tagged — N-terminal 6×His tag for IMAC purification.
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% purity verified by SDS-PAGE.
Expression systemE. coli
AccessionP00813
Protein lengthThr57-Leu363
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Predicted molecular weight 36.92 kDa
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesADA, ADA1, Adenosine aminohydrolase, Adenosine deaminase, EC:3.5.4.4
Background

Adenosine deaminase is a ~40 kDa protein. Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion.

1. Eltzschig, HK. et al. (2006) Blood 108, 1602-10. PMID: 16670267
2. Gracia, E. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1048-61. PMID: 23193172
3. Fattahi, A. et al. (2015) Urology 86, 730-4. PMID: 26166670
4. Lindley, ER. et al. (1993) The Biochemical journal 290 ( Pt 2), 457-62. PMID: 8452534
5. Jiang, C. et al. (1997) Human molecular genetics 6, 2271-8. PMID: 9361033
6. Martinez-Navio, JM. et al. (2011) Journal of leukocyte biology 89, 127-36. PMID: 20959412
7. Ginés, S. et al. (2002) The Biochemical journal 361, 203-9. PMID: 11772392
8. Gonzalez-Gronow, M. et al. (2004) The Journal of biological chemistry 279, 20993-8. PMID: 15016824
10. Li, G. et al. (2015) Pharmacology research & perspectives 3, e00121. PMID: 26038697
Note For research use only
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Formula
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