Human ADA/Adenosine deaminase Recombinant Protein (N-His-SUMO) (HF562022)

Name: Human ADA/Adenosine deaminase Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: HF562022
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HF562022
Описание	Recombinant Human ADA/Adenosine deaminase Protein, N-His-SUMO (HF562022) expressed in E. coli, spanning Thr57-Leu363. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P00813
Длина белка	Thr57-Leu363
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	46.97 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Adenosine aminohydrolase, Adenosine deaminase, ADA1, ADA
Фон	Adenosine deaminase (ADA) is a ~40 kDa protein. Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. 1. Eltzschig, HK. et al. (2006) Blood 108, 1602-10. PMID: 16670267 2. Gracia, E. et al. (2013) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 27, 1048-61. PMID: 23193172 3. Fattahi, A. et al. (2015) Urology 86, 730-4. PMID: 26166670 4. Lindley, ER. et al. (1993) The Biochemical journal 290 ( Pt 2), 457-62. PMID: 8452534 5. Jiang, C. et al. (1997) Human molecular genetics 6, 2271-8. PMID: 9361033 6. Martinez-Navio, JM. et al. (2011) Journal of leukocyte biology 89, 127-36. PMID: 20959412 7. Ginés, S. et al. (2002) The Biochemical journal 361, 203-9. PMID: 11772392 8. Gonzalez-Gronow, M. et al. (2004) The Journal of biological chemistry 279, 20993-8. PMID: 15016824 10. Li, G. et al. (2015) Pharmacology research & perspectives 3, e00121. PMID: 26038697
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ADA/Adenosine deaminase Protein, N-His-SUMO

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Даташит

Human ADA/Adenosine deaminase Recombinant Protein (N-His-SUMO).pdf

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