Human AFG3L2 Recombinant Protein (N-His) (HP047012)

Name: Human AFG3L2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP047012
Price: 211 USD
Availability: InStock

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Número de catálogo	HP047012
Descripción	Recombinant Human AFG3L2 Protein, N-His (HP047012) expressed in E. coli, spanning Lys297-Ser765. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q9Y4W6
Longitud de proteína	Lys297-Ser765
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	54.14 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	AFG3-like protein 2, AFG3L2, Paraplegin-like protein
Fondo	Mitochondrial inner membrane m-AAA protease component AFG3L2 is a ~88 kDa protein. Catalytic component of the m-AAA protease, a protease that plays a key role in proteostasis of inner mitochondrial membrane proteins, and which is essential for axonal and neuron development. AFG3L2 possesses both ATPase and protease activities: the ATPase activity is required to unfold substrates, threading them into the internal proteolytic cavity for hydrolysis into small peptide fragments. The m-AAA protease carries out quality control in the inner membrane of the mitochondria by mediating degradation of mistranslated or misfolded polypeptides. The m-AAA protease complex also promotes the processing and maturation of mitochondrial proteins, such as MRPL32/bL32m, PINK1 and SP7. Mediates protein maturation of the mitochondrial ribosomal subunit MRPL32/bL32m by catalyzing the cleavage of the presequence of MRPL32/bL32m prior to assembly into the mitochondrial ribosome. 1. Augustin, S. et al. (2009) Molecular cell 35, 574-85. PMID: 19748354 2. Tsai, CW. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 4388-4393. PMID: 28396416 3. Ding, B. et al. (2018) Biochemistry 57, 4225-4235. PMID: 29932645 4. Richter, U. et al. (2019) Life science alliance 2. PMID: 30683687 5. Puchades, C. et al. (2019) Molecular cell 75, 1073-1085.e6. PMID: 31327635 6. Liu, Y. et al. (2023) Science (New York, N.Y.) 382, 820-828. PMID: 37917749 7. Shi, X. et al. (2024) Molecular cell 84, 802-810.e6. PMID: 38157846 8. Richter, U. et al. (2015) The Journal of cell biology 211, 373-89. PMID: 26504172 10. Greene, AW. et al. (2012) EMBO reports 13, 378-85. PMID: 22354088
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human AFG3L2 Protein, N-His

Referencias

Datasheet

Human AFG3L2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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