Anti-Human AFG3L2 Polyclonal Antibody (HP047014)

Name: Anti-Human AFG3L2 Polyclonal Antibody
Brand: abinScience
SKU: HP047014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP047014

Description

Anti-Human AFG3L2 Polyclonal Antibody (HP047014) is a rabbit polyclonal antibody detecting AFG3L2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human AFG3L2 (Lys297-Ser765).

Target

AFG3-like protein 2, AFG3L2, Paraplegin-like protein

Purification

Purified by antigen affinity column.

Accession

Q9Y4W6

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Mitochondrial inner membrane m-AAA protease component AFG3L2 is a ~88 kDa protein. Catalytic component of the m-AAA protease, a protease that plays a key role in proteostasis of inner mitochondrial membrane proteins, and which is essential for axonal and neuron development. AFG3L2 possesses both ATPase and protease activities: the ATPase activity is required to unfold substrates, threading them into the internal proteolytic cavity for hydrolysis into small peptide fragments. The m-AAA protease carries out quality control in the inner membrane of the mitochondria by mediating degradation of mistranslated or misfolded polypeptides. The m-AAA protease complex also promotes the processing and maturation of mitochondrial proteins, such as MRPL32/bL32m, PINK1 and SP7. Mediates protein maturation of the mitochondrial ribosomal subunit MRPL32/bL32m by catalyzing the cleavage of the presequence of MRPL32/bL32m prior to assembly into the mitochondrial ribosome.

1. Augustin, S. et al. (2009) Molecular cell 35, 574-85. PMID: 19748354

2. Tsai, CW. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 4388-4393. PMID: 28396416

3. Ding, B. et al. (2018) Biochemistry 57, 4225-4235. PMID: 29932645

4. Richter, U. et al. (2019) Life science alliance 2. PMID: 30683687

5. Puchades, C. et al. (2019) Molecular cell 75, 1073-1085.e6. PMID: 31327635

6. Liu, Y. et al. (2023) Science (New York, N.Y.) 382, 820-828. PMID: 37917749

7. Shi, X. et al. (2024) Molecular cell 84, 802-810.e6. PMID: 38157846

8. Richter, U. et al. (2015) The Journal of cell biology 211, 373-89. PMID: 26504172

10. Greene, AW. et al. (2012) EMBO reports 13, 378-85. PMID: 22354088

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AFG3L2 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Mouse kidney lysate
Lane 2: Mouse brain lysate
Lane 3: Rat kidney lysate
Lane 4: Rat brain lysate
Lane 5: Rat liver lysate
Lane 6: Mouse cerebellum lysate
Lane 7: Rat cerebellum lysate

References

Datasheet

Anti-Human AFG3L2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-Human AFG3L2 Polyclonal Antibody (HP047014)

Overview

Images

References

Datasheet

SDS

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