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| カタログ番号 | HP047012 |
|---|---|
| 説明 |
Recombinant Human AFG3L2 Protein, N-His (HP047012) expressed in E. coli, spanning Lys297-Ser765. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9Y4W6 |
| タンパク質長 | Lys297-Ser765 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 54.14 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | AFG3-like protein 2, AFG3L2, Paraplegin-like protein |
| 背景 | Mitochondrial inner membrane m-AAA protease component AFG3L2 is a ~88 kDa protein. Catalytic component of the m-AAA protease, a protease that plays a key role in proteostasis of inner mitochondrial membrane proteins, and which is essential for axonal and neuron development. AFG3L2 possesses both ATPase and protease activities: the ATPase activity is required to unfold substrates, threading them into the internal proteolytic cavity for hydrolysis into small peptide fragments. The m-AAA protease carries out quality control in the inner membrane of the mitochondria by mediating degradation of mistranslated or misfolded polypeptides. The m-AAA protease complex also promotes the processing and maturation of mitochondrial proteins, such as MRPL32/bL32m, PINK1 and SP7. Mediates protein maturation of the mitochondrial ribosomal subunit MRPL32/bL32m by catalyzing the cleavage of the presequence of MRPL32/bL32m prior to assembly into the mitochondrial ribosome. 1. Augustin, S. et al. (2009) Molecular cell 35, 574-85. PMID: 19748354 2. Tsai, CW. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, 4388-4393. PMID: 28396416 3. Ding, B. et al. (2018) Biochemistry 57, 4225-4235. PMID: 29932645 4. Richter, U. et al. (2019) Life science alliance 2. PMID: 30683687 5. Puchades, C. et al. (2019) Molecular cell 75, 1073-1085.e6. PMID: 31327635 6. Liu, Y. et al. (2023) Science (New York, N.Y.) 382, 820-828. PMID: 37917749 7. Shi, X. et al. (2024) Molecular cell 84, 802-810.e6. PMID: 38157846 8. Richter, U. et al. (2015) The Journal of cell biology 211, 373-89. PMID: 26504172 10. Greene, AW. et al. (2012) EMBO reports 13, 378-85. PMID: 22354088 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human AFG3L2 Protein, N-His
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