Human AIFM1/AIF Recombinant Protein (N-His) (HF658012)

Name: Human AIFM1/AIF Recombinant Protein (N-His)
Brand: abinScience
SKU: HF658012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HF658012
Description	Recombinant Human AIFM1/AIF Protein, N-His (HF658012) expressed in E. coli, spanning Leu102-Asp613. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O95831
Longueur de protéine	Leu102-Asp613
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	58.1 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	AIFM1, AIF, Programmed cell death protein 8, PDCD8, Apoptosis-inducing factor 1, mitochondrial
Contexte	Apoptosis-inducing factor 1, mitochondrial (AIFM1/AIF) is a ~66 kDa protein. Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. 1. Kim, JT. et al. (2006) FEBS letters 580, 6375-83. PMID: 17094969 2. Ghezzi, D. et al. (2010) American journal of human genetics 86, 639-49. PMID: 20362274 3. Rinaldi, C. et al. (2012) American journal of human genetics 91, 1095-102. PMID: 23217327 4. Mashimo, M. et al. (2021) The Journal of biological chemistry 296, 100046. PMID: 33168626 5. Sevrioukova, IF. (2016) Journal of molecular biology 428, 3650-65. PMID: 27178839 6. Son, YO. et al. (2009) Apoptosis : an international journal on programmed cell death 14, 796-808. PMID: 19418225 7. Hangen, E. et al. (2015) Molecular cell 58, 1001-14. PMID: 26004228
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human AIFM1/AIF Protein, N-His

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Human AIFM1/AIF Recombinant Protein (N-His).pdf

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