Anti-AIFM1/AIF Polyclonal Antibody (HF658014)

Name: Anti-AIFM1/AIF Polyclonal Antibody
Brand: abinScience
SKU: HF658014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HF658014

Description

Anti-AIFM1/AIF Polyclonal Antibody (HF658014) is a rabbit polyclonal antibody detecting AIFM1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human AIFM1/AIF (Leu102-Asp613).

Target

AIFM1, AIF, Programmed cell death protein 8, PDCD8, Apoptosis-inducing factor 1, mitochondrial

Purification

Purified by antigen affinity column.

Accession

O95831

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Apoptosis-inducing factor 1, mitochondrial (AIFM1/AIF) is a ~66 kDa protein. Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. Release into the cytoplasm is mediated upon binding to poly-ADP-ribose chains. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA.

1. Kim, JT. et al. (2006) FEBS letters 580, 6375-83. PMID: 17094969

2. Ghezzi, D. et al. (2010) American journal of human genetics 86, 639-49. PMID: 20362274

3. Rinaldi, C. et al. (2012) American journal of human genetics 91, 1095-102. PMID: 23217327

4. Mashimo, M. et al. (2021) The Journal of biological chemistry 296, 100046. PMID: 33168626

5. Sevrioukova, IF. (2016) Journal of molecular biology 428, 3650-65. PMID: 27178839

6. Son, YO. et al. (2009) Apoptosis : an international journal on programmed cell death 14, 796-808. PMID: 19418225

7. Hangen, E. et al. (2015) Molecular cell 58, 1001-14. PMID: 26004228

Note

For research use only.

Images

References

Datasheet

Anti-AIFM1/AIF Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-AIFM1/AIF Polyclonal Antibody (HF658014)

Overview

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References

Datasheet

SDS

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