Human ALDH3A2 Recombinant Protein (N-His) (HW301012)

Name: Human ALDH3A2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HW301012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HW301012
Descripción	Recombinant Human ALDH3A2 Protein, N-His (HW301012) expressed in E. coli, spanning Met1-Lys428. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P51648
Longitud de proteína	Met1-Lys428
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	50.46 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	ALDH10, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, FALDH, Microsomal aldehyde dehydrogenase, Fatty aldehyde dehydrogenase, ALDH3A2
Fondo	Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) is a ~54 kDa protein. Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid. 1. Lloyd, MD. et al. (2007) Journal of enzyme inhibition and medicinal chemistry 22, 584-90. PMID: 18035827 2. Sanders, RJ. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 2064-71. PMID: 18182499 3. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490 4. Keller, MA. et al. (2014) Nature communications 5, 4439. PMID: 25047030 5. Kelson, TL. et al. (1997) Biochimica et biophysica acta 1335, 99-110. PMID: 9133646 6. Verhoeven, NM. et al. (1998) FEBS letters 429, 225-8. PMID: 9662422
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human ALDH3A2 Protein, N-His

Referencias

Datasheet

Human ALDH3A2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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