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Anti-Human ALDH3A2 Polyclonal Antibody (HW301014)

Anti-Human ALDH3A2 Polyclonal Antibody
Anti-Human ALDH3A2 Polyclonal Antibody
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Overview
Catalog No.HW301014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human ALDH3A2 (Met1-Lys428).
Target ALDH10, Aldehyde dehydrogenase family 3 member A2, Aldehyde dehydrogenase 10, FALDH, Microsomal aldehyde dehydrogenase, Fatty aldehyde dehydrogenase, ALDH3A2
Purification Purified by antigen affinity column.
Accession P51648
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Aldehyde dehydrogenase family 3 member A2 (ALDH3A2) is a ~54 kDa protein. Catalyzes the oxidation of medium and long chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. Responsible for conversion of the sphingosine 1-phosphate (S1P) degradation product hexadecenal to hexadecenoic acid.

1. Lloyd, MD. et al. (2007) Journal of enzyme inhibition and medicinal chemistry 22, 584-90. PMID: 18035827
2. Sanders, RJ. et al. (2008) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 22, 2064-71. PMID: 18182499
3. Nakahara, K. et al. (2012) Molecular cell 46, 461-71. PMID: 22633490
4. Keller, MA. et al. (2014) Nature communications 5, 4439. PMID: 25047030
5. Kelson, TL. et al. (1997) Biochimica et biophysica acta 1335, 99-110. PMID: 9133646
6. Verhoeven, NM. et al. (1998) FEBS letters 429, 225-8. PMID: 9662422
Note For research use only.
Images
  • Anti-Human ALDH3A2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ALDH3A2 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: MCF-7 cell lysate
    Lane 2: Hela cell lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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