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| カタログ番号 | HD328012 |
|---|---|
| 説明 |
Recombinant Human ALKBH2 Protein, N-His (HD328012) expressed in E. coli, spanning Ser56-Lys261. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q6NS38 |
| タンパク質長 | Ser56-Lys261 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 25.75 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ABH2, ALKBH2, Alkylated DNA repair protein alkB homolog 2, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, DNA oxidative demethylase ALKBH2, EC:1.14.11.33, Oxy DC1 |
| 背景 | DNA oxidative demethylase ALKBH2 is a ~29 kDa protein. Dioxygenase that repairs alkylated nucleic acid bases by direct reversal oxidative dealkylation. Can process both double-stranded (ds) and single-stranded (ss) DNA substrates, with a strong preference for dsDNA. Uses molecular oxygen, 2-oxoglutarate and iron as cofactors to oxidize the alkyl groups that are subsequently released as aldehydes, regenerating the undamaged bases. Probes the base pair stability, locates a weakened base pair and flips the damaged base to accommodate the lesion in its active site for efficient catalysis. Repairs monoalkylated bases, specifically N1-methyladenine and N3-methylcytosine, as well as higher order alkyl adducts such as bases modified with exocyclic bridged adducts known as etheno adducts including 1,N6-ethenoadenine, 3,N4-ethenocytosine and 1,N2-ethenoguanine. 1. Duncan, T. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 16660-5. PMID: 12486230 2. Aas, PA. et al. (2003) Nature 421, 859-63. PMID: 12594517 3. Lee, DH. et al. (2005) The Journal of biological chemistry 280, 39448-59. PMID: 16174769 4. Chen, B. et al. (2010) Molecular bioSystems 6, 2143-9. PMID: 20714506 5. Li, P. et al. (2013) Cell reports 4, 817-29. PMID: 23972994 6. Zdżalik, D. et al. (2015) DNA repair 30, 1-10. PMID: 25797601 7. Yang, CG. et al. (2008) Nature 452, 961-5. PMID: 18432238 8. Yi, C. et al. (2012) Nature structural & molecular biology 19, 671-6. PMID: 22659876 |
| 注意事項 | For research use only |
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