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| カタログ番号 | HP629012 |
|---|---|
| 説明 |
Recombinant Human AMFR Protein, N-His (HP629012) expressed in E. coli, spanning Gln456-Ser643. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9UKV5 |
| タンパク質長 | Gln456-Ser643 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 23.67 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Autocrine motility factor receptor, AMFR, E3 ubiquitin-protein ligase AMFR, RNF45, AMF receptor, gp78, RING finger protein 45 |
| 背景 | E3 ubiquitin-protein ligase AMFR is a ~72 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane. In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction. 1. Shimizu, K. et al. (1999) FEBS letters 456, 295-300. PMID: 10456327 2. Fang, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 14422-7. PMID: 11724934 3. Liang, JS. et al. (2003) The Journal of biological chemistry 278, 23984-8. PMID: 12670940 4. Pabarcus, MK. et al. (2009) Archives of biochemistry and biophysics 483, 66-74. PMID: 19103148 5. Liu, Y. et al. (2014) eLife 3, e01369. PMID: 24424410 6. Wang, YJ. et al. (2017) Nature cell biology 19, 808-819. PMID: 28604676 7. Song, BL. et al. (2005) Molecular cell 19, 829-40. PMID: 16168377 9. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human AMFR Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
