Anti-Human AMFR Polyclonal Antibody (HP629014)

Name: Anti-Human AMFR Polyclonal Antibody
Brand: abinScience
SKU: HP629014
Price: 96 USD
Availability: InStock

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Datasheet
SDS

Overview

Catalog No.

HP629014

Description

Anti-Human AMFR Polyclonal Antibody (HP629014) is a rabbit polyclonal antibody detecting AMFR in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human AMFR (Gln456-Ser643).

Target

AMFR

Purification

Purified by antigen affinity column.

Accession

Q9UKV5

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

E3 ubiquitin-protein ligase AMFR, 2.3.2.36, Autocrine motility factor receptor, AMF receptor, RING finger protein 45, gp78, AMFR, RNF45

Background

E3 ubiquitin-protein ligase AMFR is a ~72 kDa protein. E3 ubiquitin-protein ligase that mediates the polyubiquitination of lysine and cysteine residues on target proteins, such as CD3D, CYP3A4, CFTR, INSIG1, SOAT2/ACAT2 and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG1 complex at the ER membrane. In addition, interaction of AMFR with AUP1 facilitates interaction of AMFR with ubiquitin-conjugating enzyme UBE2G2 and ubiquitin ligase RNF139, leading to sterol-induced HMGCR ubiquitination. The ubiquitinated HMGCR is then released from the ER into the cytosol for subsequent destruction.

1. Shimizu, K. et al. (1999) FEBS letters 456, 295-300. PMID: 10456327

2. Fang, S. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 14422-7. PMID: 11724934

3. Liang, JS. et al. (2003) The Journal of biological chemistry 278, 23984-8. PMID: 12670940

4. Pabarcus, MK. et al. (2009) Archives of biochemistry and biophysics 483, 66-74. PMID: 19103148

5. Liu, Y. et al. (2014) eLife 3, e01369. PMID: 24424410

6. Wang, YJ. et al. (2017) Nature cell biology 19, 808-819. PMID: 28604676

7. Song, BL. et al. (2005) Molecular cell 19, 829-40. PMID: 16168377

9. Jo, Y. et al. (2013) Molecular biology of the cell 24, 169-83. PMID: 23223569

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AMFR polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate

References

Datasheet

Anti-Human AMFR Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human AMFR Polyclonal Antibody (HP629014)

Overview

Images

References

Datasheet

SDS

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