Recombinant Human ANGPTL3 Protein, C-His (HV176021) expressed in Mammalian Cells, spanning Ser17-Arg224. Purity: >90% by SDS-PAGE.
Highlights
●His-Tagged — N-terminal 6×His tag for IMAC purification.
●Mammalian Expression — Proper folding and native glycosylation.
●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión
Mammalian cells
Número de acceso
Q9Y5C1
Longitud de proteína
Ser17-Arg224
Aplicaciones
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
27.18 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
Angiopoietin-related protein 3, Angiopoietin-5, ANG-5, Angiopoietin-like protein 3, ANGPTL3(17-221), ANGPTL3(17-224), ANGPTL3, ANGPT5, UNQ153/PRO179
Fondo
Angiopoietin-related protein 3 (ANGPTL3) is a ~53 kDa protein. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids.
1. Koishi, R. et al. (2002) Nature genetics 30, 151-7. PMID: 11788823
2. Ono, M. et al. (2003) The Journal of biological chemistry 278, 41804-9. PMID: 12909640
3. Robciuc, MR. et al. (2013) Arteriosclerosis, thrombosis, and vascular biology 33, 1706-13. PMID: 23661675
4. Tikka, A. et al. (2014) Bioscience reports 34, e00160. PMID: 25495645
5. Shimizugawa, T. et al. (2002) The Journal of biological chemistry 277, 33742-8. PMID: 12097324
6. Lee, EC. et al. (2009) The Journal of biological chemistry 284, 13735-13745. PMID: 19318355
7. Liu, J. et al. (2010) The Journal of biological chemistry 285, 27561-70. PMID: 20581395
8. Shimamura, M. et al. (2007) Arteriosclerosis, thrombosis, and vascular biology 27, 366-72. PMID: 17110602
9. Shan, L. et al. (2009) The Journal of biological chemistry 284, 1419-24. PMID: 19028676
10. Shimamura, M. et al. (2003) Biochemical and biophysical research communications 301, 604-9. PMID: 12565906
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human ANGPTL3 Protein, C-His
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