Anti-ANGPTL3 Polyclonal Antibody (HV176014)

Name: Anti-ANGPTL3 Polyclonal Antibody
Brand: abinScience
SKU: HV176014
Price: 168 USD
Availability: InStock

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References
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SDS

Overview

Catalog No.

HV176014

Description

Anti-ANGPTL3 Polyclonal Antibody (HV176014) is a rabbit polyclonal antibody detecting ANGPTL3 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ANGPTL3 (Gly241-Glu460).

Target

ANG-5, Angiopoietin-like protein 3, ANGPT5, Angiopoietin-5, ANGPTL3, Angiopoietin-related protein 3

Purification

Purified by antigen affinity column.

Accession

Q9Y5C1

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Angiopoietin-related protein 3 (ANGPTL3) is a ~53 kDa protein. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake. Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity. The inhibition of LPL activity appears to be an indirect mechanism involving recruitment of proprotein convertases PCSK6 and FURIN to LPL leading to cleavage and dissociation of LPL from the cell surface; the function does not require ANGPTL3 proteolytic cleavage but seems to be mediated by the N-terminal domain, and is not inhibited by GPIHBP1. Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids.

1. Koishi, R. et al. (2002) Nature genetics 30, 151-7. PMID: 11788823

2. Ono, M. et al. (2003) The Journal of biological chemistry 278, 41804-9. PMID: 12909640

3. Robciuc, MR. et al. (2013) Arteriosclerosis, thrombosis, and vascular biology 33, 1706-13. PMID: 23661675

4. Tikka, A. et al. (2014) Bioscience reports 34, e00160. PMID: 25495645

5. Shimizugawa, T. et al. (2002) The Journal of biological chemistry 277, 33742-8. PMID: 12097324

6. Lee, EC. et al. (2009) The Journal of biological chemistry 284, 13735-13745. PMID: 19318355

7. Liu, J. et al. (2010) The Journal of biological chemistry 285, 27561-70. PMID: 20581395

8. Shimamura, M. et al. (2007) Arteriosclerosis, thrombosis, and vascular biology 27, 366-72. PMID: 17110602

9. Shan, L. et al. (2009) The Journal of biological chemistry 284, 1419-24. PMID: 19028676

10. Shimamura, M. et al. (2003) Biochemical and biophysical research communications 301, 604-9. PMID: 12565906

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ANGPTL3 polyclonal antibody at 1ug/mL on recombinant ANGPTL3 (Catalog No. HV176012).

References

Datasheet

Anti-ANGPTL3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Assay Kits

Anti-ANGPTL3 Polyclonal Antibody (HV176014)

Overview

Images

References

Datasheet

SDS

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