Human ANKRD27 Recombinant Protein (N-His) (HP196012)

Name: Human ANKRD27 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP196012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HP196012
Описание	Recombinant Human ANKRD27 Protein, N-His (HP196012) expressed in E. coli, spanning Met654-Ser902. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q96NW4
Длина белка	Met654-Ser902
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	28.99 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	ANKRD27, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein
Фон	Ankyrin repeat domain-containing protein 27 (ANKRD27) is a ~116 kDa protein. May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics. May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation. Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7. Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38. Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1. 1. Zhang, X. et al. (2006) Journal of cell science 119, 1053-62. PMID: 16525121 2. Wang, F. et al. (2008) Biochemical and biophysical research communications 372, 162-7. PMID: 18477474 3. Schäfer, IB. et al. (2012) Nature structural & molecular biology 19, 1300-9. PMID: 23104059 4. Burgo, A. et al. (2012) Developmental cell 23, 166-80. PMID: 22705394 5. Burgo, A. et al. (2009) EMBO reports 10, 1117-24. PMID: 19745841 6. Hesketh, GG. et al. (2014) Developmental cell 29, 591-606. PMID: 24856514
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human ANKRD27 Protein, N-His

Ссылки

Даташит

Human ANKRD27 Recombinant Protein (N-His).pdf

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Formula

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C₁ (Stock Conc.)

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