Anti-Human ANKRD27 Polyclonal Antibody (HP196014)

Name: Anti-Human ANKRD27 Polyclonal Antibody
Brand: abinScience
SKU: HP196014
Price: 168 USD
Availability: InStock

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References
Datasheet
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Overview

Catalog No.

HP196014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human ANKRD27 (Met654-Ser902).

Target

ANKRD27, Ankyrin repeat domain-containing protein 27, VPS9 domain-containing protein

Purification

Purified by antigen affinity column.

Accession

Q96NW4

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ankyrin repeat domain-containing protein 27 (ANKRD27) is a ~116 kDa protein. May be a guanine exchange factor (GEF) for Rab21, Rab32 and Rab38 and regulate endosome dynamics. May regulate the participation of VAMP7 in membrane fusion events; in vitro inhibits VAMP7-mediated SNARE complex formation by trapping VAMP7 in a closed, fusogenically inactive conformation. Involved in peripheral melanosomal distribution of TYRP1 in melanocytes; the function, which probably is implicating vesicle-trafficking, includes cooperation with Rab32, Rab38 and VAMP7. Involved in the regulation of neurite growth; the function seems to require its GEF activity, probably towards Rab21, and VAMP7 but not Rab32/38. Proposed to be involved in Golgi sorting of VAMP7 and transport of VAMP7 vesicles to the cell surface; the function seems to implicate kinesin heavy chain isoform 5 proteins, GOLGA4, RAB21 and MACF1.

1. Zhang, X. et al. (2006) Journal of cell science 119, 1053-62. PMID: 16525121

2. Wang, F. et al. (2008) Biochemical and biophysical research communications 372, 162-7. PMID: 18477474

3. Schäfer, IB. et al. (2012) Nature structural & molecular biology 19, 1300-9. PMID: 23104059

4. Burgo, A. et al. (2012) Developmental cell 23, 166-80. PMID: 22705394

5. Burgo, A. et al. (2009) EMBO reports 10, 1117-24. PMID: 19745841

6. Hesketh, GG. et al. (2014) Developmental cell 29, 591-606. PMID: 24856514

Note

For research use only.

Images

References

Datasheet

Anti-Human ANKRD27 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human ANKRD27 Polyclonal Antibody (HP196014)

Overview

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References

Datasheet

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